Characterization of Odontoblasts in Supernumerary Tooth-derived Dental Pulp Stem Cells between Passages by Real-Time PCR
지상은, 송솔, 이준행, 김종빈, 김종수,
소속 상세정보
지상은 ( Ji Sang-Eun ) - Cheonan Ebargae Orthodontic Clinic
송솔 ( Song Sol ) - Dankook University College of Dentistry Department of Pediatric Dentistry
이준행 ( Lee Joon-Haeng ) - Dankook University College of Dentistry Department of Pediatric Dentistry
김종빈 ( Kim Jong-Bin ) - Dankook University College of Dentistry Department of Pediatric Dentistry
김종수 ( Kim Jong-Soo ) - Dankook University College of Dentistry Department of Pediatric Dentistry
Abstract
The aim of this study is to compare the properties of odontoblast gene of early passage cells and late passage cells derived from impacted maxillary supernumerary teeth. Impacted supernumerary teeth with maxilla were extracted from 12 patients (8 males, 4 females) between 6 - 9 years old without medical history. Real-time polymerase chain reaction (PCR) was conducted to compare characterization of odontoblast cell in the 3rd and 10th passage, and between with bone inducing additive group and without additive group. Genes for odontoblasts characteristics are osteonectin (ONT), alkaline phosphatase (ALP), osteocalcin (OCN), dentin matrix protein 1 (DMP-1) and dentin sialophosphoprotein (DSPP). The level of gene expression was in a decreasing order of ONT, ALP, OCN, DMP-1 and DSPP in the 3rd passage, and in decreasing order of ONT, DMP-1, OCN, ALP, and DSPP in the 10th passage in the undifferentiation and differentiation group. The order of ONT, DMP-1, and OCN did not changed. ALP and DMP-1 were switched in order. ALP and DMP-1 may be used as important markers for differentiating between the 3rd passage and 10th passage cells. Considering that supernumerary tooth was extracted young age and the time required to cultured 10th passage was short, supernumerary tooth can be considered a useful donor site of dental pulp stem cells.
키워드
Odontoblast; Supernumerary tooth; Dental pulp stem cell; Real time polymerase chain reaction
원문 및 링크아웃 정보
등재저널 정보